Anisakis simplex (s.l.) resistance to the action of gastric enzymes depends upon previous treatments applied to infected fish mince and affects antigen release

Background Freezing is considered the most suitable technological treatment to avoid Anisakis infection from eating raw or undercooked fish but modifications of their cuticles upon freezing may reduce resistance gastric fluids, provoking a greater release allergens. This work aimed study relationship between freezing-induced simplex s.l., antigen recognition, and oral digestion in spiked mince. Results (i) Differences non-treated larvae that survived / thawing were studied terms respiratory capacity, survival simulated fluid (SGF), recognition antigens (ii) Untreated (i.e. chilled) mince containing live larvae, frozen at two rates, with negative (uninfected) positive (infected broken larvae) as controls, subjected phases process. able survive showed lower faster mortality compared controls). released significantly more than freeze-surviving only after 96 h SGF. In treatments rendering complete mortality, highest loss integrity was found fast freezing. There correlation number ruptures phase, whereas complex trend observed plus phases. Conclusion These results suggest new factor consider for sensitized patients numbers L3 should be reduced before industrial minimize risk. © 2020 Society Chemical Industry